From freeze to function: optimised cryopreservation and mitochondrial analysis workflow for skeletal muscle biopsies

HIGHLIGHTS

• What: The authors focused on quantifying artefacts and functional organelles that provide insight into muscle physiology. By providing practical solutions to data_analysis challenges, this study provides a framework for a more accurate assessment of skeletal muscle samples in both research and diagnostic contexts, ultimately contributing to advancements in understanding muscle bioenergetics and pathology. The study demonstrates that the isopentane/histocassette combination uniquely provides artefact-free preservation across the entire tissue section, making it the most effective method for cryopreservation of skeletal muscle biopsies (Fig 7). Given the particular difficulties in handing skeletal muscle biopsies, with their unique . . .
  

   

  

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