Functional analyses of resurrected and contemporary enzymes illuminate an evolutionary path for the emergence of exolysis in polysaccharide lyase family 2*

HIGHLIGHTS

• who: Richard McLean from the Products were analyzed directly or following a, fold concentration by TLC (as above) or high performance anion exchange with pulsed amperometric detectionThis analysis was performed with a Dionex , chromatography system (Thermo Scientific) equipped with an autosampler as well as a pulsed amperometric detector for total carbohydrates and a UV-visible detector for unsaturated galacturonides. Aqueous sample (typically , ␮l) was injected into an analytical (, ⫻, mm) CarboPac , column (Thermo Scientific) and eluted at a, .4-ml min⫺, flow rate with a sodium acetate gradient (, min, mM have published the research work: Functional Analyses of . . .
  

   

  

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