Functional analysis of disulfide linkages clustered within the amino terminus of human apolipoprotein b*

HIGHLIGHTS

• who: Khai Tran from the Materials-Restriction and DNA modification enzymes and endoglycosidase H were purchased from New England BiolabsProMixy (a mixture of [ S]methionine and [ S]cysteine, Ci/mmol), horseradish peroxidase-conjugated antibody to mouse immunoglobulin G, and the enhanced chemiluminescence (ECLy) reagents for immunoblotting were obtained from Amersham Pharmacia Biotech. Protease inhibitors , and, acetyl-leucyl-leucyl-norleucinal and sheep anti-human apoB antiserum were obtained from Boehringer Mannheim. Monoclonal antibodies , and , were gifts of R. Milne and Y. Marcel (Ottawa Heart Institute), S. Young (Gladstone Institute), and L. Curtiss (Scripps Research Institute). Monoclonal antibody , for . . .
  

   

  

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