Functional expression of the nitrogenase fe protein in transgenic rice

HIGHLIGHTS

• who: Can Baysal from the University of Madrid, Spain have published the article: Functional expression of the nitrogenase Fe protein in transgenic rice, in the Journal: (JOURNAL) of 20/04/2022
• what: The yield of NbNifHHt was ~5-6 mg kg-1 leaf tissue using STAC purification but the activity of the as-isolated enzyme was low (maximum 25 nmol ethylene min-1 mg-1 when NifH was used at a 40-fold molar ratio to NifDK).
• how: Here the authors used the TS-tag and STAC to purify NifHHt from stably transformed . . .
  

   

  

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