HIGHLIGHTS
- who: Marija Tesic from the Yeast Strains and Growth Conditions-Standard genetic techniques and growth media were used in the studyYeast transformations were performed using the lithium acetate method (30), and bacterial transformations by electroporation were done using Gene Pulser (Bio-Rad). Geldanamycin was the gift of the Drug Synthesis and Chemistry Branch, Developmental Therapeutics Program, Division of Cancer Treatment, NCI (National Institutes of Health). Alpha factor was the gift of L. Hicke (Northwestern University, Evanston, IL). Cyclosporin A was purchased from Affinity Bioreagents. Strains used in this study are shown in Table I. MTY189h was generated . . .
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