Functional relevance of urinary-type plasminogen activator receptor-α3β1 integrin association in proteinase regulatory pathways*

HIGHLIGHTS

• who: Supurna Ghosh from the EXPERIMENTAL PROCEDURES Materials-Plasminogen and plasmin were purified by affinity chromatography from outdated human plasma as described previously (23)Anti-human integrin ␣, and ␤, monoclonal antibodies (P , and P, ) were obtained from Chemicon (Temecula, CA). Affinity-purified polyclonal antibody specific for phosphorylated p42/p, mitogen-activated protein kinase (anti-ACTIVE威 MAPK p42/p, or ERK1/2) was purchased from Promega (Madison, WI). Anti-ERK1/, (anti-p42/p44) antibodies, which recognize both phosphorylated and nonphosphorylated p42/p44, were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Phospho-Src (Tyr416) antibody was obtained from Cell . . .
  

   

  

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