Gdesigner: computational design of synthetic grnas for cas12a-based transcriptional repression in mammalian cells

HIGHLIGHTS

• who: Michael A. Crone from the (UNIVERSITY) have published the research: gDesigner: computational design of synthetic gRNAs for Cas12a-based transcriptional repression in mammalian cells, in the Journal: (JOURNAL) of 28/04/2022
• what: By co-transfecting all combination of reporters and transcription factors into HEK293FT_cells (9 u00d7 9 matrix), the authors show that the repressors are largely orthogonal (Fig 3c, Supplementary Fig 3).
• how: The authors designed and tested four promoter configurations with gRNAs facing inwards outwards 5` or 3` direction in HEK293FT_cells (Supplementary Fig 1a). Inclusion and ethics statement Research was . . .
  

   

  

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