Generation of synthetic antibody fragments with optimal complementarity determining region lengths for notch-1 recognition

HIGHLIGHTS

  • who: Notch signaling and colleagues from the National Institutes of Health (NIH), United States Medical University of Vienna, Austria have published the paper: Generation of synthetic antibody fragments with optimal complementarity determining region lengths for Notch-1 recognition, in the Journal: (JOURNAL)
  • how: During the course of the Fab generation the authors showed that screening focused diversity libraries implementing next-generation sequencing (NGS) approaches and fine-tuning the library diversity can improve binding solutions for Notch-1 recognition. The authors used NGS information for identifying and reconstructing low-frequency clones with lessfrequent CDRH3 lengths for . . .

     

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