Genome editing in primary cells and in vivo using viral-derived nanoblades loaded with cas9-sgrna ribonucleoproteins

HIGHLIGHTS

• who: Philippe E. Mangeot from the Centre de Médecine Lyon Est, Lyon, France have published the research work: Genome editing in primary cells and in vivo using viral-derived Nanoblades loaded with Cas9-sgRNA ribonucleoproteins, in the Journal: NATURE COMMUNICATIONS NATURE COMMUNICATIONS of September/22,/2010
• how: For this the authors developed an approach similar to that described by Fu and colleagues19 by creating a series of HEK293T reporter cell lines transduced with different versions of a GFP transgene bearing silent point mutations located in the sgRNA target site (Fig 3b right panel).
 



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