Genomic sequence capture of plasmodium relictum in experimentally infected birds

HIGHLIGHTS

• who: Vincenzo A. Ellis from the We used c, ng of DNA isolated from the blood of infected birds for sequence capture following the approach detailed in Huang et_al [23]. DNA was quantified using a Qubit, ., Fluorometer (Thermo Scientific, Waltham, MA, USA). We sheared the DNA to c., bp fragments using a, u2122 Focused-ultrasonicator (Covaris, MA, USA) and then proceeded to perform the sequence capture protocol outlined by Agilent Technologies for the S u00ad ureSelectXT kit. We incubated the probes with samples for , h and we used , cycles of PCR in the post-capture amplification with . . .
  

   

  

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