Glaucocalyxin a exerts anticancer effect on osteosarcoma by inhibiting gli1 nuclear translocation via regulating pi3k/akt pathway

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  • who: Jianwei Zhu from the Male BALB/c nude mice (35-40-day-old), weighing, g, were purchased from the Comparative Medicine Centre of Yangzhou UniversityThe animal study was carried out according to National Institutes of Health regulations and approved by the Institutional Animal Care and Use Committee. The mice were maintained in a pathogen-free environment (, ± , °C and , ±, % humidity) on a , h light and , h dark cycle with food and water supplied freely during the entire experiment. On day, ×, HOS cells suspended in , μl PBS were subcutaneously inoculated in the right flank of each nude mice . . .

     

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