HIGHLIGHTS
SUMMARY
Dulbecco`s modified Eagle`s culture medium (DMEM) enriched with glutamax I, trypsin/ethylene diamine tetraacetic acid (EDTA), penicillin/streptomycin solution and fetal bovine serum (FBS), used during in_vitro studies, were purchased from GIBCO (Life Technologies, Monza, Italy). The Netherlands) provided the cellulose acetate membrane used for in_vitro release studies (cut-off 10 kDa). In detail, the calibration curve of the drug was obtained using various dilutions (140-5 µg/mL) of a stock solution of DOX (1 mg/mL in water). The second step of this investigation was based on the evaluation of . . .
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