Glutaredoxin 1 up-regulates deglutathionylation of α4 integrin and thereby restricts neutrophil mobilization from bone marrow

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  • who: Yuanyuan You from the Cell culture , cells were purchased from the ATCC (CCL- TM) and cultured in RPMI , medium (Gibco) supplemented with , mM, glutamine, % heat-inactivated fetal bovine serum (Gibco), and, % penicillin/streptomycind, cells were treated with, .3% DMSO for , days in Iscove's modified Dulbecco's medium to be differentiated to a neutrophillike phenotype. HUVECs were purchased from the ATCC (CRL- TM) and maintained in Dulbecco's modified Eagle's medium (Gibco) supplemented with, % fetal bovine serum and, % penicillin/streptomycin. HEK T cells were obtained from the ATCC (CRL- TM) and cultured in Dulbecco's . . .

     

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