Gly-103 in the n-terminal domain of saccharomyces cerevisiae rad51 protein is critical for dna binding*

HIGHLIGHTS

• who: Xiao-Ping Zhang from the Yeast Media, Strains, and Plasmids-Standard yeast media were used for cell growthS. cerevisiae WDHY1930, a rad, deletion mutant (his3-⌬, leu2-3, trp, ura3, pep4, rad51-⌬::KanMX), was created by using pFA-Kan, to replace the , open reading frame from amino acids, by the KAN resistance marker and used as a host for overexpression of wild type and mutant Rad, proteins (55). A Rad G, expressing plasmid was generated by in vitro mutagenesis of plasmid pR51., (kind gift of Patrick Sung, Yale University (56)), in which the constitutive phosphoglycerol kinase promoter . . .
  

   

  

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