Golgi stress induces sirt2 to counteract shigella infection via defatty-acylation

HIGHLIGHTS

• who: Miao Wang from the Intraperitoneal shigellosis mice model study The Sflexneri intraperitoneal infection mouse study was done as previously described50. Briefly, week old wildtype and , knockout, /B J mice (Jackson Lab, Sirt2tm1. Fwa) were intraperitoneally administrated with , million Shigella per , g of mouse body weight. After , h, mice were sacrificed, and tissue were harvested. The peritoneal wash was also collected. Tissues were washed in PBS with gentamycin (, u03bcg/ml) to remove superficial bacteria, rinsed twice in antibiotic-free PBS, and then mechanically homogenized in PBS with, .5% Triton , (, mg of tissue per ml of PBS). The . . .
  

   

  

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