HIGHLIGHTS
SUMMARY
The second group comprises middle-weight solutes such as large peptides, proteins, and lipoproteins (e_g, b2-microglobulin, parathyroid hormone, cytokines, etc.). Conventionally, liquid chromatography-tandem mass spectrometry (LC-MS/MS) represents the main diagnostic system in the measurement of IS thanks to its elevated performance. In recent years, several working groups have improved the LC-MS/MS method for the quantification of blood IS. The mixture was held forfor 1515 min atat 7070 Indigo byboth bothLC-MS/MS LC-MS/MSand andTheremino. The authors confirmed the formation of Indigo with the derivatization reaction of . . .
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