H protocol marker for oxidative stress in cultured cells

HIGHLIGHTS

  • who: OH and collaborators from the Centre for Analysis and Synthesis, Department of Chemistry, Lund University, , Lund, Sweden have published the Article: H Protocol Marker for Oxidative Stress in Cultured Cells, in the Journal: Metabolites 2019, 45 of /2019/
  • what: A standardised protocol is needed for sample preparation, with the overall goal to make glutathione concentrations comparable .
  • how: For instant measurement of the redox state in the cell the authors used NEM in combination with acetonitrile for immediate deproteinisation to block all enzymatic activities and to mask the thiol groups. For this the . . .

     

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