High dietary fat selectively increases catalase expression within cardiac mitochondria*

HIGHLIGHTS

• who: Paul M. Rindler from the the University of MichiganPPARA⫺/⫺ global knock-out mice were purchased from The Jackson Laboratory (stock, ). At the designated time points, mice were sacrificed by cervical dislocation, and the hearts removed for analysis. Approximately , mg of heart tissue was removed from the apex for RNA extraction as described below. The remaining tissue was homogenized in buffer A (buffer A: , mM MOPS, mM EDTA, mM mannitol, mM sucrose, pH, .4). These whole heart lysates were cleared by centrifugation at , ⫻ g for , min and decanted through cheesecloth. Protein concentrations were determined using the BCA . . .
  

   

  

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