High-level raav vector production by radv-mediated amplification of small amounts of input vector

HIGHLIGHTS

• who: Stefan Weger from the Plasmid pTR-, [30] carrying a CMV enhancer/promoter driven GFP cassette flanked by the , ITR`s was kindly provided by Nick Muzyczka (University of Florida)The plasmids pShuttle-p5-Rep and pShuttle-p5-sRep containing the , p, promoter and the complete Rep, coding region have been described [29]. The term sRep denotes a Rep, sequence with recoding of , nt , to , according to [27]. For generation of pShuttle-MMTV-Rep and pShuttle-MMTV-sRep, the sequences between the BglII site preceding the p, promoter and the unique SalI site within the Rep-ORF . . .
  

   

  

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