High-resolution epitope mapping and affinity binding analysis comparing a new anti-human lag3 rabbit antibody clone to the commonly used mouse 17b4 clone

HIGHLIGHTS

  • who: P. Daniel Warren and collaborators from the Roche Tissue Diagnostics Ventana, Tucson, AZ, USA have published the research work: High-Resolution Epitope Mapping and Affinity Binding Analysis Comparing a New Anti-Human LAG3 Rabbit Antibody Clone to the Commonly Used Mouse 17B4 Clone, in the Journal: Antibodies 2022, 60 of 16/08/2022
  • what: The authors demonstrate the application of immunogen fragmentation and alanine-scanning mutagenesis to fine-map the minimum epitope sequences of both clones using the orthogonal technologies of Western blot, automated capillary electrophoresis (ACE), SPR, and IHC. The work demonstrates the . . .

     

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