High resolution respirometry of isolated mitochondria from adult octopus maya (class: cephalopoda) systemic heart

HIGHLIGHTS

SUMMARY

    High resolution respirometry method for mitochondria of Octopus maya heart proteins, amino_acids, and purine biosynthesis) besides synthesizing most of the adenosine_triphosphate (ATP) through oxidative_phosphorylation (OXPHOS) to supply the energy demands of the different aerobic tissues. Currently, new methods have been used that allow evaluating mitochondrial respiratory states in isolated mitochondria, permeabilized cells in homogenized tissue, in intact cells and in tissue sections. Mitochondrial research has benefited from the availability of organelle preparations isolated from tissues, specifically those procedures to isolate mitochondria based on differential_centrifugation, which allows separating cell constituents based on their different sedimentation . . .

     

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