Histidine 268 in 3-deoxy-d-arabino-heptulosonic acid 7-phosphate synthase plays the same role as histidine 202 in 3-deoxy-d-manno-octulosonic acid 8-phosphate synthase*

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• who: David L. Howe from the SCHEME , IncThe Promega Wizard DNA purification kit was utilized for plasmid isolation and purification. The E. coli cells, XL1-Blue, and the QuikChange mutagenesis kit were obtained from Stratagene Cloning Systems. E. coli cells, BL21(DE3), were obtained from Novagen. Thermal cycling was performed using an MJR Research thermal cycler. DNA sequencing and primer synthesis was performed by the University of Michigan Biomedical Resources Core Facility. PEP mono(cyclohexyl)ammonium salt, E, P sodium salt, , dithiobis-(2-nitrobenzoic acid) (DTNB), and diethyl pyrocarbonate (DEPC) were obtained from Sigma Chemical Co. The . . .
  

   

  

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