Histone chaperone asf1 acts with rif1 to promote dna end joining in brca1-deficient cells

HIGHLIGHTS

• who: Mengfan Tang from the Cell lines T cells were purchased from the American Type Culture Collection and cultured in Dulbecco`s modified Eagle`s medium (DMEM) supplemented with, % fetal bovine serumRPE1-hTERT FLAG-Cas, TP53-/- BRCA1-/- cells were a gift kindly provided by Dr Daniel Durocher (University of Toronto) and were cultured in DMEM with, % fetal bovine serum. Inducible , KO HeLa cells were cultured in DMEM with, % fetal bovine serum. Plasmids DNA fragments corresponding to sgRNAs were cloned into p, (Addgene:, ) for the knock-in of, terminal SFB tag at endogenous , locus. LentiCRISPRv, (Addgene:, ) was used . . .
  

   

  

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