Hiv-1 triggers wave2 phosphorylation in primary cd4 t cells and macrophages, mediating arp2/3-dependent nuclear migration*

HIGHLIGHTS

• who: Mark Spear from the EXPERIMENTAL PROCEDURES Isolation of Resting , T Cells and Monocytes from Peripheral Blood-All protocols involving human subjects were reviewed and approved by the George Mason University Institutional Review BoardResting , T cells were purified from peripheral blood of , negative donors by two rounds of negative selection as previously described (16). Briefly, for the first-round depletion, we used monoclonal antibodies against human CD14, and HLA-DR, DP, and DQ (BD Biosciences). For the second-round depletion, we used monoclonal antibodies against human CD8, CD11b, and , (BD Biosciences). Antibody-bound cells were depleted by . . .
  

   

  

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