Hook2, a microtubule-binding protein, interacts with par6α and controls centrosome orientation during polarized cell migration

HIGHLIGHTS

• who: Emilie Pallesi-Pocachard from the Developmental Biology Institute have published the paper: Hook2, a microtubule-binding protein, interacts with Par6α and controls centrosome orientation during polarized cell migration, in the Journal: Scientific Reports Scientific Reports
• how: For the yeast two-hybrid assay the authors used L40 yeast and a LexA system with human Hook2 Cter (amino_acids from 438 to 719 a BamHI/BamHI fragment) in pBTM116 as bait and human PAR6α​WT PAR6α​ Nter PAR6α​PDZ PAR6α​ Δ​PDZ PAR6 Cter inserted in the pACT2 vector (BD Biosciences) as target of interaction have already been . . .
  

   

  

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