HIGHLIGHTS
SUMMARY
In this context, the authors tried to determine the impact of selecting this artificial medium, largely employed in research, in comparison to human plasma and how the choice of culturing and stimulating a single lymphocyte population (CD4+ T_cells) as opposed to bulk PBMCs influences CD4+ T_cell gene_expression profiles. Total HIV DNA load in CD4+ T_cells was quantified for all the individuals and was 255, 35, and 377 HIV-1 DNA copies per million CD4+ T_cells for S1, S2, and S3, respectively. An aliquot of non-cultured CD4+ T_cells (T0) was collected as a control . . .
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