HIGHLIGHTS
SUMMARY
Mycoplasma detection on AD-MSCs, which is mandatory for in-house in_vivo studies, was performed using the nested PCR Takara Mycoplasma Detection Kit (Takara Bio Inc. For the xenotransplant experiments, one out of four biological samples was considered also accounting for the complexity of in_vivo combining human AFT with autologous 4 of 17 AD-MSCs that were additionally gene modified by GFP. AT was processed and isolated cells were amplified until P2 and cryopreserved for the in_vivo implantation together with the autologous fat obtained from the same patient during the scheduled abdominal liposuction. After . . .
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