Human fibroblasts facilitate the generation of ipscs-derived mammary-like organoids

HIGHLIGHTS

• who: Xueqin Dai from the Fibroblast-derived iPSCs lines were generous donated by professor Duanqin Pei`s Lab (Westlake University)The BT fibroblasts isolated from human skin tissues were reprogramed to obtain iPSCs, which were further characterized by AP staining, immunofluorescence, bisulfate sequencing, karyotyping, and teratomas formation test [26]. iPSCs were maintained using Matrigel (#35427, BD) matrix and mTe, medium (#85851, Stem cell) that needed replaced fresh medium daily. HFF (human foreskin fibroblasts) (obtained from ATCC, CRL-2429) were maintained in DMEM (C BT, Gibco) supplemented with, % fetal bovine serum (10099-141, Gibco). The iPSCs were dissociated . . .
  

   

  

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