HIGHLIGHTS
SUMMARY
Human milk proteins, peptides and free amino_acids (AAs) support adequate growth and immune functions. HM-type III was assigned if LNFP II and LNDFH I were below Determination of fatty_acids and amino_acids in human milk Human milk samples were thawed overnight at 4◦ C and then gently vortexed and aliquoted. Free amino_acids Most free amino_acid levels were significantly different depending on lactation period. HMOs, fatty_acids, and amino_acid levels differed largely from Western cohorts. Third, significant differences in HMOs, fatty_acids, and amino_acids (total and free) were observed across lactational periods among the different HM-types . . .

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