Human ran cysteine 112 oxidation by pervanadate regulates its binding to keratins*

HIGHLIGHTS

• who: Guo-Zhong Tao from the cells are cultured in the presence of PV and proteasome inhibitors but not in the presence of PV alone (Fig C). This explains why PV-oxidized Ran is readily seen in cell-free systems (Fig., ) but is difficult to detect in cells. Notably, calpain (PD150606) and lysosome (pepstatin/leupeptin) inhibitors had a minimal effect on, kDa Ran turnover (not shown). These findings are consistent with earlier studies that demonstrate accumulation of oxidized proteins and a concomitant decline in proteolytic activity with age (35, ). PV-induced Oxidation of Ran Cys, Accounts for . . .
  

   

  

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