HIGHLIGHTS
- who: Oscar Vargas-Rodriguez from the (The Genomics Shared Resource at the Ohio State University Comprehensive Cancer Center)Preparation of tRNAs and aminoacyl-tRNA substrates All tRNA variants used in these studies were prepared by in vitro transcription as previously described (14). Briefly, for each tRNA, a linear DNA template for , RNA polymerase was obtained by Bst, (NEB) digestion of a plasmid carrying the corresponding gene. Transcribed tRNA was purified using denaturing, % PAGE. To ensure high yields of tRNA variants with cytidine at position, a hammerhead ribozyme was inserted at , of the tRNA gene (42). A plasmid . . .
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