Hybrid tetramers of porcine liver fructose-1,6-bisphosphatase reveal multiple pathways of allosteric inhibition*

HIGHLIGHTS

• who: Scott W. Nelson from the Materials-F, F, NADP⫹, and AMP were purchased from SigmaGlucose-6-phophate dehydrogenase and phosphoglucose isomerase came from Roche Molecular Biochemicals. Other chemicals were of reagent grade or the equivalent. QSW-HR HPLC resin was from TosoHass Bioseparations. The FBPase-deficient Escherichia coli strain DF , came from the Genetic Stock Center at Yale University. Plasmids used in the expression of wild-type and Tyr, Trp FBPases came from a previous investigation (17, ). Sigma Genosys provided all oligonucleotides used in directed mutations. Mutagenesis of Wild-Type FBPase-Mutations were accomplished by deletion . . .
  

   

  

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