Hydrogen peroxide activates nf-ùºb through tyrosine phosphorylation of iùºbù± and serine phosphorylation of p65

HIGHLIGHTS

• who: Yasunari Takada from the Reagents-Bacteria-derived human rTNF, purified to homogeneity with a specific activity of , ⫻ , units/mg, was kindly provided by Genentech (South San Francisco, CA)Penicillin, streptomycin, Iscove's modified Dulbecco's medium, RPMI , medium, fetal bovine serum, and LipofectAMINE , were obtained from Invitrogen. H , was obtained from Sigma. Antibodies anti-p65, anti-p50, antiI␬B␣, anti-cyclin, and anti-phosphotyrosine (PY99) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Phospho-specific anti-I␬B␣ (Ser-32) antibody was purchased from Cell Signaling (Beverly, MA). Anti-IKK-␣ and anti-IKK-␤ antibodies were . . .
  

   

  

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