Identification and characterization of a novel human matrix metalloproteinase with unique structural characteristics, chromosomal location, and tissue distribution*

HIGHLIGHTS

• who: Alberto M. Pendás from the Materials-A human liver cDNA library constructed in lgt, and two Northern blots containing polyadenylated RNAs from different human tissues were purchased from Clontech (Palo Alto, CA)Oligonucleotides were synthesized in an Applied Biosystems (Foster City, CA) model A DNA synthesizer. Restriction endonucleases and other reagents used for molecular cloning were from Boehringer Mannheim (Mannheim, Germany). Double-stranded DNA probes were radiolabeled with [ P]dCTP (, Ci/mmol) from Amersham Corp. (Amersham, UK) using a commercial random priming kit purchased from Pharmacia Biotech Inc. (Uppsala, Sweden). Screening of a Human Liver . . .
  

   

  

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