Identification of a binding site on soluble rankl that can be targeted to inhibit soluble rank-rankl interactions and treat osteoporosis

HIGHLIGHTS

• who: Dane Huang from the female C BL/, mice by flushing the marrow from the femur and tibia, then cultured in u03b1-MEM complete medium in presence of , ng/mL, CSFAfter , h, BMMs were adherent and can be used for osteoclastogenesis assay. RAW264., cells stably transfected with an NF-u03baBdriven or NFATc, luciferase (kindly providing , cells by professor Jiake Xu in University of Western Australia) which used for reported gene assays were cultured in u03b1-MEM complete medium. HEK T cells (ATCC, Cat# CRL-3216) used in transfecting RANK plasmid and expressing RANK inclusion body were cultured . . .
  

   

  

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