Identification of a novel type of focal adhesion remodelling via fak/frnk replacement, and its contribution to cancer progression

HIGHLIGHTS

• who: Masatsune Tsujioka from the Frnk-KO mice were generated using the CRISPR/Cas, system by referring to a previous study that generated Frnk-KO mice by targeted disruption of the Frnk promoter [19]hCas, mRNA and sgRNAs were generated using pT7-hCas, and pT7-sgRNA plasmids (kindly provided by Dr. M. Ikawa, Osaka University), respectively [21]. After digestion of pT7-hCas, with EcoRI, hCas, mRNA was synthesized using an in vitro RNA transcription kit (mMESSAGE mMACHINE , Ultra Kit, Ambion), according to the manufacturer`s instructions. Two pairs of forward and reverse oligos targeting the, u2032-untranslated . . .
  

   

  

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