Identification of amino acid sequence in the hinge region of human vitamin d receptor that transfers a cytosolic protein to the nucleus*

HIGHLIGHTS

• who: Toshimi Michigami from the Plasmid Constructions of VDR-GFP Fusion Proteins-The expression vector of the human VDR cDNA, pSG5-hVDR was kindly provided by DrM. R. Haussler (University of Arizona) (35). Although the amino acid number is indicated according to the original paper concerning the human VDR cDNA (GenBankTM accession number, ), the expression vector pSG5-hVDR itself contains the VDR cDNA, which starts from the second translation start point due to the existence of polymorphism, resulting in the , amino acids (hVDR amino acids , , ). GFP fusion vector pGreen Lantern was purchased from Life Technologies, Inc. To . . .
  

   

  

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