Identification of d-arabinan-degrading enzymes in mycobacteria

HIGHLIGHTS

• who: Omar Al-Jourani from the RNA sequencing Bcellulosilyticus was cultured in defined media (Table, ) containing , mg ml, AG or glucose, in triplicate , ml cultures. Cells were harvested at mid-log phase and stored in RNA protect (Qiagen). RNA was purified with the RNAeasy Kit. Prior to library preparation, rRNA was depleted using the Pan-Prokaryote riboPOOLs kit (siTOOLs Biotech). In brief, u00b5g of total RNA was incubated for , min at , u00b0C and , min at , u00b0C with , pmol of rRNA-specific biotinylated DNA probes in, ., mM Tris-HCl pH, .5,mM EDTA, and , mM NaCl. DNA-rRNA . . .
  

   

  

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