Identification of genomic variants of sars-cov-2 using nanopore sequencing

HIGHLIGHTS

SUMMARY

During the last two years, the COVID-19 pandemic has been the main concern worldwide, causing socio-economical losses at an unanticipated scale. The authors have compared the Nanopore sequencing results obtained using the V3 and V4 primer pools, and evaluated their quality as compared to Ion Torrent sequencing on an S5 instrument. The authors used a shorter, adapted version of the SARS-CoV-2 sequencing protocol (nCoV2019 sequencing protocol v3 (LoCost) V.3) developed and adapted by the ARTIC Network (https://artic.network/) using the reagent from New England BioLabs (NEB, Ipswich, MA . . .

 



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