Identification of small molecule inhibitors of jumonji at-rich interactive domain 1b (jarid1b) histone demethylase by a sensitive high throughput screen*

HIGHLIGHTS

• who: Joyce Sayegh from the (UNIVERSITY) have published the paper: Identification of Small Molecule Inhibitors of Jumonji AT-rich Interactive Domain 1B (JARID1B) Histone Demethylase by a Sensitive High Throughput Screen*, in the Journal: (JOURNAL) of March/29,/2013
• what: The authors first characterized full-length JARID1B.
• how: These results showed that the Km for bio-H3K4me3 is ⬃15 nM and the Km for ␣-KG is ⬃5 ␮M. Final screening conditions for JARID1B were 4 nM enzyme 64 nM bio-H3K4me3 peptide 50 ␮M Fe(II) 10 ␮M ␣-KG and 100 ␮M ascorbate . . .
  

   

  

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