Identification of two entamoeba histolyticasequence-specific ure4 enhancer-binding proteins with homology to the rna-binding motif rrm*

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• who: Joanna M. Schaenman from the PAGE gels by trypsinization were microsequenced by tandem mass spectrometry by the WM. Keck Biomedical Mass Spectrometry Laboratory at the University of Virginia. Five to six amino acids from tryptic peptide sequences were used to design degenerate PCR primers. The forward primer used for amplification of Eh, was TA(T/C)GA(T/ C)GTIGTIGA(A/G)GC(T/A/C)AC, based on the peptide sequence YDVVEAT(I/L)VK). The forward primer used for amplification of Eh, was CCAACIGAATT(C/T)TC(A/T)GA(C/T)GA, based . . .
  

   

  

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