Identification of vp1 peptides diagnostic of encephalomyocarditis virus from swine

HIGHLIGHTS

• who: Encephalomyocarditis virus (EMCV and colleagues from the EMCV isolate , (GenBank Accession NoHM641897) used in this study was isolated and kept in our lab (Submitted, JUL-2010). , cells were kept in Dulbecco's Modified Eagle Medium supplemented with, % fetal bovine serum. The SP2/, cells were grown in RPMI , Medium supplemented with, % fetal bovine serum. Female BALB/c mice, weeks of age, were purchased from the Laboratory Animal Centre of Nantong University. HAT and HT Media Supplement (50×) were purchased from Sigma. Horseradish peroxidase (HRP)-tagged goat anti-mouse IgG was purchased from Wuhan Boster Biological Technology Co . . .
  

   

  

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