Immunological correlates of protection following vaccination with glucan particles containing cryptococcus neoformans chitin deacetylases

HIGHLIGHTS

• who: Ruiying Wang from the Chemicals and reagents Chemical reagents were purchased from Thermo Fisher (Pittsburgh, PA), unless otherwise specifiedMedia for culturing Cryptococcus were either Sabouraud dextrose agar, or yeast extract-peptone-dextrose (YPD), containing Difco yeast extract, Bacto peptone, and dextrose with and without, % agar. Bovine serum albumin was added to X phosphate buffered saline (PBS) at a concentration of, .5% as FACS buffer for flow cytometry staining. The complete medium for mouse cell culture was RPMI , supplemented with, % fetal bovine serum (FBS), % HEPES, % GlutaMAX, and, % Penicillin-Streptomycin. Where indicated, ., u00b5g/ml amphotericin B was included . . .
  

   

  

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