Impaired mitochondrial oxidative metabolism in skeletal progenitor cells leads to musculoskeletal disintegration

HIGHLIGHTS

• who: Chujiao Lin from the eosin (HandE) or safranin OAlternatively, intact hindlimbs containing bones and skeletal muscles were immediately fixed in ice-cold, % paraformaldehyde solution for one day for histological analysis of skeletal muscles. Semi-decalcification was carried out for five days in, ., M EDTA pH, ., at , u00b0C with constant shaking (age u2265, week), and infiltration was followed with a mixture of, % sucrose phosphate buffer for one day. All samples were embedded in a, mixture of, % sucrose solution and OCT compound (Sakura) and cut into , u03bcm thick cross sections using a cryostat (Leica Microsystems, USA). Immunofluorescence staining . . .
  

   

  

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