Importance of membrane structural integrity for rpe65 retinoid isomerization activity*

HIGHLIGHTS

• who: Marcin Golczak from the NaCl (PBS) containing , mM EDTA, resuspended in , ml of the, ray source APS, ID-D Temperature , K same buffer, and aliquoted into, ␮l samples prior to adding Wavelength , Å bis(maleimido)hexane (BMH) cross-linker (Pierce) up to, ., Space group , Unit cell constants a ⫽ b ⫽, .36, c ⫽Å mM delivered in, ., ␮l of N, dimethylformamide. The crossResolution , to, ., Å (1., toÅ) linking reaction was carried out at room temperature for , Unique reflections observed, Completeness, % (92%) min. Samples then were quenched with , mM ␤-mercaptoethMultiplicity, ., (3.2) anol and analyzed by SDS-PAGE and immunoblots with either . . .
  

   

  

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