Improved cytometric analysis of untouched lung leukocytes by enzymatic liquefaction of sputum samples

HIGHLIGHTS

  • who: Giulia Santopolo from the Research Institute of Balearic Islands (IdISBa), Palma, Spain have published the research: Improved cytometric analysis of untouched lung leukocytes by enzymatic liquefaction of sputum samples, in the Journal: (JOURNAL)
  • what: Methods: Here the authors propose an enzymatic method that rapidly liquefies samples by means of generating O u00ad 2 bubbles with endogenous catalase. This study aims to demonstrate the suitability of a novel enzymatic method to prepare respiratory samples for single-cell analysis by flow cytometry.
  • how: The authors used turbidimetry to compare the liquefaction degree and cell . . .

     

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