Improved immunoassay sensitivity and specificity using single-molecule colocalization

HIGHLIGHTS

• who: Amani A. Hariri from the (UNIVERSITY) have published the paper: Improved immunoassay sensitivity and specificity using single-molecule colocalization, in the Journal: (JOURNAL) of 23/Dec/2021
• what: Using TNF-u03b1, the authors show that SiMCA achieves a three-fold lower limit of detection compared to conventional single-color assays and exhibits consistent performance for assays performed in complex specimens such as serum and blood. The authors demonstrate that SiMCA can overcome the confounding effects of non-specific background to enable accurate, sensitive, and reproducible detection of picomolar protein concentrations even in highly complex . . .
  

   

  

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