HIGHLIGHTS
- who: Hendrik R. Sikkema from the DepartmentUniversity of have published the Article: In silico method for selecting residue pairs for single-molecule microscopy and spectroscopy, in the Journal: Scientific Reports Scientific Reports of 17/02/2021
- what: The authors aimed to find new residue pairs for smFRET that do not interfere with the docking of OpuAC. The authors propose to lower the restraints when the number of pairs is too low, but the labelled protein should always be tested for functionality.
SUMMARY
μL column volume of Ni2+-Sepharose resin was equilibrated . . .
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