In situ and in silico kinetic analyses of programmed cell death-1 (pd-1) receptor, programmed cell death ligands, and b7-1 protein interaction network

HIGHLIGHTS

• who: Kaitao Li from the Cells and proteins CHO cells (ATCC) were cultured in RPMI , (Cellgro) supplemented with, % FBS (Cellgro), units/ml penicillin, ␮g/ml streptomycin, mM, glutamine, and , mM HEPES and transfected with pc, encoding hPD-1, mPD-1, or hB7, using nucleofection (Lonza). To generate stable cell lines, transfected cells were subjected to , selection (0., mg/ml) and multiple rounds of FACS sorting for uniform surface receptor expression. CHO mB7, cells were a generous gift of Dr. Periasamy Selvaraj (Emory University, Atlanta, GA). His6-tagged and biotinylatable proteins hPD-L1, hPD-L2, mPD-L1, and . . .
  

   

  

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