In vitro kinase assay reveals adp-heptose-dependent alpk1 autophosphorylation and altered kinase activity of disease-associated alpk1 mutants

HIGHLIGHTS

  • who: Diego Garcu00eda-Weber from the Universitu00e9 have published the research: In vitro kinase assay reveals ADP-heptose-dependent ALPK1 autophosphorylation and altered kinase activity of disease-associated ALPK1 mutants, in the Journal: Scientific Reports Scientific Reports
  • how: Altogether these results showed that the mechanism of ADPH sensing that occurs during bacterial infection induces the phosphorylation of both TIFA and ALPK1. Data showed that T2 T12 and T19 are also weakly phosphorylated by ALPK1.
  • future: More work will also be needed to identify the residues that are autophosphorylated in response to ALPK1 activation . . .

     

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